agxАндрология и генитальная хирургияAndrology and Genital Surgery2070-97812412-890210.17650/2070-9781-2014-2-48-57agx-95Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL REPORTХарактеристика состояния сперматогенеза у мужчин с бесплодием, имеющих различные типы делеций AZFc-регионаCharacteristics of spermatogenesis in infertile men with the AZFc region deletionsЧерныхВячеслав БорисовичChernykhV. B.

Москва

Moscow

chernykh@med-gen.ruРудневаС. А.RudnevaS. A.

Москва

Moscow

chernykh@med-gen.ruСорокинаТ. М.SorokinaT. M.

Москва

Moscow

chernykh@med-gen.ruШилейкоЛ. В.ShileykoL. V.

Москва

Moscow

chernykh@med-gen.ruКурилоЛ. Ф.KuriloL. F.

Москва

Moscow

chernykh@med-gen.ruРыжковаО. П.RyzhkovaO. P.

Москва

Moscow

chernykh@med-gen.ruЧухроваА. Л.ChukhrovaA. L.

Москва

Moscow

chernykh@med-gen.ruПоляковА. В.PolyakovA. V.

Москва

Moscow

chernykh@med-gen.ruФГБУ «Медико-генетический научный центр» РАМНРоссияMedical Genetics Research Centre, Russian Academy of Medical SciencesRussian Federation2014041220141524857Copyright © Черных В.Б., Руднева С.А., Сорокина Т.М., Шилейко Л.В., Курило Л.Ф., Рыжкова О.П., Чухрова А.Л., Поляков А.В., 20142014Черных В.Б., Руднева С.А., Сорокина Т.М., Шилейко Л.В., Курило Л.Ф., Рыжкова О.П., Чухрова А.Л., Поляков А.В.Chernykh V.B., Rudneva S.A., Sorokina T.M., Shileyko L.V., Kurilo L.F., Ryzhkova O.P., Chukhrova A.L., Polyakov A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://agx.elpub.ru/jour/article/view/95

Исследовано состояние сперматогенеза у 218 российских мужчин с бесплодием, имеющих различные делеции AZFc-региона Y-хромосомы. Выявлено явное различие полных и частичных AZFc-делеций по структуре форм патозооспермии и концентрации сперматозоидов. Средние значения концентрации сперматозоидов для носителей делеций b2/b4, gr/gr и b2/b3 составили 0,37 ± 0,13; 12,2 ± 7,1 и 30,3 ± 5,3 млн/мл соответственно. Выраженное угнетение сперматогенеза, характеризующееся наличием секреторной азооспермии и олигозооспермии тяжелой степени, обнаружено у 93, 42 и 57 % мужчин с делециями b2/b4, b2/b3 и gr/gr соответственно. При количественном кариологическом анализе незрелых половых клеток из осадка эякулята выявлены признаки нарушения деления и созревания незрелых половых клеток: от частичного блока на допахитенных стадиях профазы I мейоза и/или делений созревания вплоть до полного угнетения сперматогенеза. Более мягкие нарушения сперматогенеза, характеризующиеся наличием олигозооспермии среднетяжелой или легкой степени либо астенозооспермии и/или тератозооспермии, отмечены у 7; 20; 30 % и 0; 38; 10 % носителей делеций b2/b4, b2/b3 и gr/gr соответственно.

Spermatogenetic defects were analyzed in the cohort of 218 russian infertile men with various AZFc region deletions of the Y chromosome. Clear differences were found in both the percentage of pathozoospermia forms and sperm concentration between infertile men with complete (b2/b4) and partial (b2/b3 and gr/gr) AZFc deletions. Sperm concentration in the carriers of b2/b4, gr/gr and b2/b3 deletions, were 0.37 ± 0.13, 12.2 ± 7.1 and 30.3 ± 5.3 mln/ml, respectively. Severe spermatogenesis defects were detected in 93, 42 and 57 % patients with b2/b4, b2/b3 and gr/gr deletions, respectively. Quantitative karyological analysis of immature germ cells from ejaculate sediment revealed from incomplete spermatogenesis arrest at prepaсhytene stages to complete spermatogenesis depletion. Moderate oligozoospemia and/or astheno-, teratozoospermia were found in 7; 20; 30 %; and 0; 38; 10 % of the carriers of b2/b4, b2/b3 and gr/gr deletions, respectively.

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The authors declare that there are no conflicts of interest present.