agxАндрология и генитальная хирургияAndrology and Genital Surgery2070-97812412-890210.17650/2070-9781-2013-2-40-45agx-42Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL REPORTБиологическая безопасность образцов (сперматозоидов, ооцитов и эмбрионов человека) при длительном хранении в жидком азотеBiosafety samples (sperm, oocytes and embryos person) during prolonged storage in liquid nitrogenПетроваЕ. В.PetrovaYe. V.

Отделение вспомогательных технологий в лечении бесплодия, Москва

Department of Assistive Technology in the Treatment of Infertility, Moscow

kurilo@med-gen.ruМакароваН. П.MakarovaN. P.

Отделение вспомогательных технологий в лечении бесплодия, Москва

kurilo@med-gen.ruКурилоЛюбовь ФедоровнаKuriloL. F.

лаборатория генетики нарушений репродукции, Москва

Laboratory of Genetics of Reproduction Disorders, Moscow

kurilo@med-gen.ruФГБУ «Научный центр акушерства, гинекологии и перинатологии им. В.И. Кулакова» Минздрава РоссииРоссияAcad. V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of RussiaRussian FederationФГБУ «Медико-генетический научный центр» РАМНРоссияMedical Genetics Research Center, Russian Academy of Medical SciencesRussian Federation2013281120141424045Copyright © Петрова Е.В., Макарова Н.П., Курило Л.Ф., 20142014Петрова Е.В., Макарова Н.П., Курило Л.Ф.Petrova Y.V., Makarova N.P., Kurilo L.F.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://agx.elpub.ru/jour/article/view/42

Криоконсервация сперматозоидов, ооцитов и эмбрионов играет важную роль в современных вспомогательных репродуктивных технологиях. На сегодняшний день единственным доступным источником низких температур, удовлетворяющим условиям долгосрочного хранения клеток, является жидкий азот. Однако необходимо учитывать, что существует вероятность передачи инфекционных агентов между образцами через жидкий азот при их совместном размещении в криохранилище. В статье обсуждаются пути снижения риска перекрестной контаминации и комплексный подход к обеспечению биологической безопасности процесса криоконсервации в рамках создания криобанков.

Cryopreservation of sperm, oocyte and embryo plays an important role in modern assisted reproductive technologies. To date the only available source of low temperatures satisfying the conditions of long-term storage of cells is liquid nitrogen. However there is a possibility of transmission of infectious agents between samples in liquid nitrogen at their co-location in a cryostorage. The article discusses ways to reduce the risk of cross-contamination and the integrated approach to biosecurity.

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The authors declare that there are no conflicts of interest present.